Design of mixing pulses for NMR spectroscopy by repeated rotating frames / Coote, Paul William
- Bib ID:
- 7124222
- Format:
- Book and Microform
- Author:
- Coote, Paul William, author
- Description:
-
- Ann Arbor : ProQuest Dissertations & Theses, 2014
- 177 p.
- ISBN:
- 9781321015201
- Summary:
-
Finally, a selection of temporally orchestrated mixing experiments, in which pulses with different mixing properties are applied in sequence to generate novel correlation patterns, are demonstrated. These patterns are potentially highly informative for protein spectroscopy, and cannot be observed using standard mixing pulses.
In protein NMR spectroscopy, homonuclear mixing pulses are used to reveal correlations amongst chemically bonded nuclear spins. These pulses must have low RF power levels and short durations to avoid probe damage and sample heating. However, standard mixing pulses require high RF power to cover the large bandwidths of chemical shift frequencies encountered in practice. This motivates the design of new mixing pulses which have high bandwidth-to-power ratios. Such pulses are especially useful for experiments at high Zeeman field, on the carbon channel, and/or with long mixing duration. This thesis presents a new way to design homonuclear mixing pulses that are broadband, narrowband, or multi-band, to suit various existing and new experiments in protein NMR spectroscopy. These pulses are designed analytically, rather than by numerical optimization, by iterative construction of a series of nutating frames of reference.
Pulse parameters are chosen frame-by-frame to effectively compress the chemical shift bandwidth arbitrarily many times, while largely maintaining couplings between spins. This means that the effective Hamiltonian is dominated by spin-spin couplings, rather than mismatched chemical shift frequencies, and therefore magnetization will move throughout the network of interacting nuclei. This design methodology is explored analytically, via simulation, and in experiments. Pulses are created which have higher bandwidth than currently available broadband mixing pulses. Robustness to inhomogeneity in the pulse amplitude, which is important for the pulses to perform reliably in practice, is demonstrated. The loss of signal due to relaxation effects under the new pulses is no worse than under widely-used existing pulses. Novel multi-band pulses, which save power by neglecting unpopulated spectral regions, were created and successfully implemented.
- Notes:
-
- Advisors: Gerhard Wagner Committee members: Roger Brockett; Navin Khaneja; Yue Lu.
- Source: Dissertation Abstracts International, Volume: 75-10(E), Section: B.
- English
- Subject:
- Other authors/contributors:
- Harvard University. Engineering and Applied Sciences, degree granting institution
- Copyright:
-
In Copyright
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Copyright status was determined using the following information:
- Material type:
- Literary Dramatic Musical
- Published status:
- Published
- Publication date:
- 2014
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