Ion-pair chromatography of nucleic acid constituents in blood (Australian marsupials, hplc) [microform]
- Bib ID:
- 3287497
- Format:
- Book and Microform
- Author:
- Perrone, Pamela Ann
- Description:
- 180 p.
- Summary:
-
Among Australia's many attributes is the fact that it is the homeland of the marsupials. Research, ranging from studies on their eating and mating habits to the measurement of several blood parameters such as electrolyte and enzyme levels, have been conducted in order to learn more about this group of animals. However, more information was necessary to better understand the phylogeny of the species and certain biochemical observations such as the very high levels of acid phosphatase in the Tasmanian devil. Therefore the reverse-phase high-performance liquid chromatographic (RP-HPLC) plasma profiles of several Australian marsupials were obtained. The nucleoside, base and aromatic amino acid profiles of five marsupial species were compared and identified. One of the features that permitted the uniequivocal identification of the Tasmanian devil from the other species was the difference in areas over the first seven-and-half minutes of the chromatogram between species.
In this early portion of the chromatogram are weakly retained and non-retained (ionic or polar) compounds. To determine what ionic compounds might be present in this time segment. The technique of ion-pair chromatography (IPC) was investigated. A literature review of the application of IPC to nucleotides, nucleosides and bases is provided; few applications and thorough investigations have been reported. Hence, a study on the optimization of IPC conditions was conducted. The variables of type, size and concentration of counter-ion, pH of the mobile phase, temperature, type and percent organic modifier and stationary phase have been explored. Due to the complexity of the plasma/serum matrix, peak identification is a difficult task. Generally to confirm an identity, data is gathered from numerous detectors such as the UV (peak height ratios and scans) and fluoresence, and from chemical reactions and enzymatic peak shifts.
Another potentially useful on-line detector is the electrochemical (EC) detector which measures the ability of the compound to undergo oxidation or reduction. A preliminary investigation of the utility of EC detection to the nucleotides, nucleosides and bases is reported.
- Notes:
-
- (UnM)AAI8401417
- Source: Dissertation Abstracts International, Volume: 44-10, Section: B, page: 3072.
- Thesis (Ph.D.)--University of Rhode Island, 1983.
- Reproduction:
- Microfiche. Ann Arbor, Mich.: University Microfilms International.
- Subject:
- Chemistry, Analytical
- Other authors/contributors:
- University of Rhode Island
- Copyright:
-
In Copyright
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Copyright status was determined using the following information:
- Material type:
- Literary Dramatic Musical
- Published status:
- Unpublished
- Creation date:
- 1983
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